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proteome profiler human phospho kinase array kit  (R&D Systems)


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    R&D Systems proteome profiler human phospho kinase array kit
    Proteome Profiler Human Phospho Kinase Array Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 829 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+phospho/Proteome+Profiler+Human+Phospho-Kinase+Array+Kit/pm42031710-119-7-13
    Average 96 stars, based on 829 article reviews
    proteome profiler human phospho kinase array kit - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Recombinant nucleic acid construct
    Article Snippet: Blots are stripped and re-probed with anti-Tie-2 antibody (R&D systems) to detect total Tie-2. .. For quantitative measurement two different ELISAs are performed measuring human Tie-2 or Phospho-Tie-2 using the human Tie-2 DuoSet ELISA kit or human-Phospho-Tie-2 DuoSet IC ELISA according to the manufactures (e.g. both ELISA-Kits from R&D systems) protocol. ..

    Article Title: Ang2 antibodies
    Article Snippet: After the incubation time, medium may be quickly and fully removed from the cells, and cells may be lysed in 60 μl per well of cold 1× Tris Lysis Buffer (Meso Scale Discovery #R60TX; 150 mM NaCl, 20 mM Tris pH 7.5, 1 mM EDTA, 1 mM EGTA, 1% Triton X-100) which may contain freshly added protease and phosphatase inhibitors (1× protease inhibitor cocktail, Sigma #P8340; 1× phosphatase inhibitor cocktail 2, Sigma #P5726; 1× phosphatase inhibitor cocktail 3, Sigma #P0044; 1 mM final activated sodium orthovanadate (EMD Chemicals #567540)). .. Plates may then be placed on ice for 10 minutes, after which they may be placed on an orbital shaker at low speed for 25 minutes at 4° C. The plates may then be sealed and frozen at −80° C. The day before analysis for phospho-Tie-2 (with a human phospho-Tie-2 DuoSet ELISA kit from R&D Systems, #DYC2720), high binding ELISA plates (Greiner BioOne, #655081) may be coated overnight at 4° C. with 4 μg/ml mouse anti-human total Tie-2 capture antibody in 1×ELISA coating buffer (Surmodics/BioFX Labs #COAT-1000-01). ..

    Article Title: Preclinical validation of a novel metastasis‐inhibiting Tie1 function‐blocking antibody
    Article Snippet: .. For phospho‐Tie2 analysis, serum‐starved HUVECs were pretreated with Tie1‐binding antibodies (10 μg/ml) for 1 h. Thereafter, cells were stimulated with recombinant rhANG1 (R&D, 923‐AN) at 400 ng/ml for 15 min. HUVEC lysates were analyzed using the human phospho‐Tie‐2 DuoSet IC ELISA Kit (DYC2720, RnD). ..

    Binding Assay:

    Article Title: Ang2 antibodies
    Article Snippet: After the incubation time, medium may be quickly and fully removed from the cells, and cells may be lysed in 60 μl per well of cold 1× Tris Lysis Buffer (Meso Scale Discovery #R60TX; 150 mM NaCl, 20 mM Tris pH 7.5, 1 mM EDTA, 1 mM EGTA, 1% Triton X-100) which may contain freshly added protease and phosphatase inhibitors (1× protease inhibitor cocktail, Sigma #P8340; 1× phosphatase inhibitor cocktail 2, Sigma #P5726; 1× phosphatase inhibitor cocktail 3, Sigma #P0044; 1 mM final activated sodium orthovanadate (EMD Chemicals #567540)). .. Plates may then be placed on ice for 10 minutes, after which they may be placed on an orbital shaker at low speed for 25 minutes at 4° C. The plates may then be sealed and frozen at −80° C. The day before analysis for phospho-Tie-2 (with a human phospho-Tie-2 DuoSet ELISA kit from R&D Systems, #DYC2720), high binding ELISA plates (Greiner BioOne, #655081) may be coated overnight at 4° C. with 4 μg/ml mouse anti-human total Tie-2 capture antibody in 1×ELISA coating buffer (Surmodics/BioFX Labs #COAT-1000-01). ..

    Phospho-proteomics:

    Article Title: Anti-vascular endothelial growth factor therapy-induced glioma invasion is associated with accumulation of Tie2-expressing monocytes
    Article Snippet: We used human MMP9 and MMP2 Quantikine ELISA kits (R&D System) for quantification according to the manufacturer's protocol. .. The Tie2 phosphorylation level in THP-1 cells exposed to normoxia or hypoxia in the presence or absence of IL4 and IL13 was determined using the human phospho-Tie-2 (Y992) cell-based ELISA (R&D) as per the company's procedure. ..

    In-Cell ELISA:

    Article Title: Anti-vascular endothelial growth factor therapy-induced glioma invasion is associated with accumulation of Tie2-expressing monocytes
    Article Snippet: We used human MMP9 and MMP2 Quantikine ELISA kits (R&D System) for quantification according to the manufacturer's protocol. .. The Tie2 phosphorylation level in THP-1 cells exposed to normoxia or hypoxia in the presence or absence of IL4 and IL13 was determined using the human phospho-Tie-2 (Y992) cell-based ELISA (R&D) as per the company's procedure. ..

    Protein-Protein interactions:

    Article Title: Kayadiol exerted anticancer effects through p53-mediated ferroptosis in NKTCL cells
    Article Snippet: .. To elucidate the signaling pathways, a proteome profiler for human phospho-kinases (ARY003C, R&D Systems, Minneapolis, MN, USA) was used. .. The levels of phosphorylation or expression were quantified using ImageJ software.

    Article Title: The serine proteases dipeptidyl-peptidase 4 and urokinase are key molecules in human and mouse scar formation
    Article Snippet: Quantification analysis was performed with the Volume tool in ImageLab 6.0.1 (Bio-Rad), adjusted to GAPDH expression, and normalized to respective Ctrl samples to calculate fold change to Ctrl. .. To analyze signaling pathways, we used a proteome profiler for human phospho-kinases (ARY003C, R&D Systems, Biotechne, Minneapolis, MN, USA) according to the manufacturer’s instructions. ..

    Article Title: Single cell landscape of hypertrophic scars identifies serine proteases as key regulators of myofibroblast differentiation
    Article Snippet: Signals were developed with SuperSignal West Dura substrate (Thermo Fisher Scientific) and imaged with a Gel Doc XR+ device (Bio-Rad Laboratories, Inc.). .. To analyse signaling pathways, we used a proteome profiler for human phospho-kinases (ARY003C, R&D Systems, Biotechne, Minneapolis, MN, USA) according to the manufacturer’s instructions. .. Human pro-collagen Ia1 ELISA (R&D Systems) and human fibronectin ELISA (R&D Systems) were performed with supernatants of TGFβ 1-stimulated FBs according to the manufacturer’s manual.

    other:

    Article Title: Vasculotide, an Angiopoietin-1 Mimetic, Restores Microcirculatory Perfusion and Microvascular Leakage and Decreases Fluid Resuscitation Requirements in Hemorrhagic Shock
    Article Snippet: 1 XXX 2017 H EMORRHAGIC shock is a life-threatening condition often followed by multiple organ failure and associated with unfavorable patient outcome.1 Microcirculatory perfusion is essential for the delivery of oxygen and nutrients to the organs, and disturbances of microcirculatory perfusion seem to have a predictive value in the development of multiple organ failure.2,3 Despite stabilization of the macrocirculation, microcirculatory hypoperfusion persists for days in hemorrhagic shock patients3 and seems to be a key mediator in the development of multiple organ failure and unfavorable patient outcome.2,3 Current treatment of hemorrhagic shock combines early control of bleeding, maintenance of critical tissue perfusion, correction of coagulopathy, and management of the systemic inflammatory response syndrome.4,5 Resuscitation with fluids and/or blood products is the first step to correct hypoperfusion and prevent subsequent organ failure.5,6 However, crystalloids have poor plasma-expanding capacities, of which What We Already Know about This Topic

    Recombinant:

    Article Title: Preclinical validation of a novel metastasis‐inhibiting Tie1 function‐blocking antibody
    Article Snippet: .. For phospho‐Tie2 analysis, serum‐starved HUVECs were pretreated with Tie1‐binding antibodies (10 μg/ml) for 1 h. Thereafter, cells were stimulated with recombinant rhANG1 (R&D, 923‐AN) at 400 ng/ml for 15 min. HUVEC lysates were analyzed using the human phospho‐Tie‐2 DuoSet IC ELISA Kit (DYC2720, RnD). ..



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    CSPG4 blockade reduces TcdB‐induced <t>pSTAT3</t> nuclear translocation in enteric glial cells. (A) Representative photomicrographs of pSTAT3 (green) immunostaining, phalloidin (red) as a cytoplasm staining, and DAPI (blue) as a nuclear staining in enteric glial cells exposed to TcdA (50 ng/mL) or TcdB (1 ng/mL) at 18 h incubation in the presence of isotype control or anti‐CSPG4 antibodies (0.001 μg/mL). Scale bars: 50 μm. (B) Percentage of enteric glial cells ( n = 5, mean ± SEM) with positive nuclear pSTAT3 staining at 18 h of incubation with TcdA and TcdB in the presence of isotype control or anti‐CSPG4 antibodies (0.001 μg/mL). One‐way ANOVA followed by the Tukey test.
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    CSPG4 blockade reduces TcdB‐induced <t>pSTAT3</t> nuclear translocation in enteric glial cells. (A) Representative photomicrographs of pSTAT3 (green) immunostaining, phalloidin (red) as a cytoplasm staining, and DAPI (blue) as a nuclear staining in enteric glial cells exposed to TcdA (50 ng/mL) or TcdB (1 ng/mL) at 18 h incubation in the presence of isotype control or anti‐CSPG4 antibodies (0.001 μg/mL). Scale bars: 50 μm. (B) Percentage of enteric glial cells ( n = 5, mean ± SEM) with positive nuclear pSTAT3 staining at 18 h of incubation with TcdA and TcdB in the presence of isotype control or anti‐CSPG4 antibodies (0.001 μg/mL). One‐way ANOVA followed by the Tukey test.
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    Image Search Results


    CSPG4 blockade reduces TcdB‐induced pSTAT3 nuclear translocation in enteric glial cells. (A) Representative photomicrographs of pSTAT3 (green) immunostaining, phalloidin (red) as a cytoplasm staining, and DAPI (blue) as a nuclear staining in enteric glial cells exposed to TcdA (50 ng/mL) or TcdB (1 ng/mL) at 18 h incubation in the presence of isotype control or anti‐CSPG4 antibodies (0.001 μg/mL). Scale bars: 50 μm. (B) Percentage of enteric glial cells ( n = 5, mean ± SEM) with positive nuclear pSTAT3 staining at 18 h of incubation with TcdA and TcdB in the presence of isotype control or anti‐CSPG4 antibodies (0.001 μg/mL). One‐way ANOVA followed by the Tukey test.

    Journal: The FASEB Journal

    Article Title: CSPG4 Mediates Inflammatory, Cell Death, and Senescence Responses in Enteric Glia Exposed to Clostridioides difficile Toxins

    doi: 10.1096/fj.202600333R

    Figure Lengend Snippet: CSPG4 blockade reduces TcdB‐induced pSTAT3 nuclear translocation in enteric glial cells. (A) Representative photomicrographs of pSTAT3 (green) immunostaining, phalloidin (red) as a cytoplasm staining, and DAPI (blue) as a nuclear staining in enteric glial cells exposed to TcdA (50 ng/mL) or TcdB (1 ng/mL) at 18 h incubation in the presence of isotype control or anti‐CSPG4 antibodies (0.001 μg/mL). Scale bars: 50 μm. (B) Percentage of enteric glial cells ( n = 5, mean ± SEM) with positive nuclear pSTAT3 staining at 18 h of incubation with TcdA and TcdB in the presence of isotype control or anti‐CSPG4 antibodies (0.001 μg/mL). One‐way ANOVA followed by the Tukey test.

    Article Snippet: After washing, cells were incubated overnight at 4°C with primary antibodies against CSPG4 (Abcam, ab275024, 1:400), nectin‐3 (PVRL3, Invitrogen, PA5‐51095, 1:200), LRP1 (Abcam, ab92544, 1:100), NFκB p65 (Santa Cruz Biotechnology, sc‐372, 1:100), or pSTAT3 (R&D Systems, AF4607, 1:100).

    Techniques: Translocation Assay, Immunostaining, Staining, Incubation, Control